The Tissue Culture Laboratory of the Ministry of Agriculture is located at the Union Agricultural Station and is a sub-unit of the Propagation Division. Plant Tissue Culture is a collective term referring to procedures used to maintain and grow plant cells and organs in vitro (in glass) under aseptic (free from mico-organisms) conditions.
It is a tool used by biotechnologists, chemists, biologits, pathologists and many other scientists in the following applications:
Secondary metabolite production
The principal objective of the Tissue Culture Unit is to strengthen the propagation capability of the Ministry of Agriculture and the wider agricultural sector through micro-propagation and germplasm maintenance of species of economic and ecological importance.
Procedures Conducted at the Lab
Parent Plant Selection and preparation -
The parent plant is the material from which plant parts will be removed to start the cultures.
Establishment of Cultures -
Small plant parts (explants) are placed in synthetic growth medium under aseptic conditions.
Explants previously established in culture are transferred from one medium or container to another into fresh medium. Subculturing is done for multiplication in vitro germplasm conservation or plantlet.